6JBF

Crystal structure of the bacterial ribosomal decoding site in complex with 4'-deoxy-4'-fluoro neamine analog (axial 4'-F)

Replaces:  5BWS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-09
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	27.111, 81.324, 50.131
Unit cell angles	90.00, 95.28, 90.00

Refinement procedure

Resolution	42.543 - 2.600
R-factor	0.1613
Rwork	0.154
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j7t
RMSD bond length	0.009
RMSD bond angle	1.053
Data scaling software	XSCALE
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.543	42.543	2.660
High resolution limit [Å]	2.600	11.610	2.600
Rmerge	0.056	0.022	0.281
Rmeas	0.061	0.024	0.305
Number of reflections	6744	83	493
<I/σ(I)>	25.15	52.12	7.63
Completeness [%]	99.8	98.8	98.6
Redundancy	6.782	6.169	6.85
CC(1/2)	0.999	1.000	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, NaCl, spermine tetrahydrochloride, sodium cacodylate