6J74

Complex of GGTaseIII and full-length Ykt6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	119.218, 119.218, 212.577
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.540 - 3.212
R-factor	0.2393
Rwork	0.237
R-free	0.28510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6J6X and 3BW6
RMSD bond length	0.002
RMSD bond angle	0.461
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.270
High resolution limit [Å]	3.210	3.210
Number of reflections	25733	1240
<I/σ(I)>	15.5
Completeness [%]	99.7
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.18M K2HPO4, 0.82M NaH2PO4