6J6O

Crystal structure of diamondback moth ryanodine receptor phosphorylation domain(2836-3050)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	55.994, 55.994, 132.598
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.666 - 1.848
R-factor	0.1904
Rwork	0.186
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4etv 5c30
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.848	5.020	1.850
Rmerge	0.068	0.031	0.715
Rmeas	0.072	0.033	0.754
Rpim	0.023	0.011	0.238
Number of reflections	21391	1204	1049
<I/σ(I)>	4.7
Completeness [%]	100.0	99.5	100
Redundancy	9.6	8.8	9.9
CC(1/2)		0.999	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298.15	1.8M Ammonium citrate tribasic pH7.0