6J6L

Crystal structure of mouse Ryanodine Receptor 2 SPRY1 Domain (650-844) disease mutant I784F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	52.945, 64.375, 108.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.860 - 1.452
R-factor	0.1731
Rwork	0.172
R-free	0.20380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c33
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.480
High resolution limit [Å]	1.450	3.940	1.450
Rmerge	0.159	0.080	0.702
Rmeas	0.173	0.087	0.824
Rpim	0.069	0.034	0.418
Number of reflections	66033	3577	3128
<I/σ(I)>	4.5
Completeness [%]	99.6	99.7	95.5
Redundancy	6	6.1	3.4
CC(1/2)		0.995	0.394

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M sodium acetate (PH4.5), 0.8 M sodium sulphate.