6J4S
Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 74.198, 111.523, 127.151 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.304 - 2.800 |
R-factor | 0.1749 |
Rwork | 0.172 |
R-free | 0.22710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j4o |
RMSD bond length | 0.009 |
RMSD bond angle | 1.015 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.147 | 1.203 |
Rpim | 0.048 | 0.505 |
Number of reflections | 14701 | 1410 |
<I/σ(I)> | 15.8 | |
Completeness [%] | 99.7 | 97.4 |
Redundancy | 10.3 | 6.3 |
CC(1/2) | 0.563 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.8 | 293 | full-length V2-SVBP complex at a concentration of 15 mg/mL was crystallized in 1.8 M sodium/potassium phosphate, pH 6.8 |