6J44
Crystal structure of the redefined DNA-binding domain of human XPA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.28240 |
Spacegroup name | P 3 |
Unit cell lengths | 66.982, 66.982, 35.462 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.460 - 2.060 |
R-factor | 0.2039 |
Rwork | 0.202 |
R-free | 0.23920 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.372 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.130 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.125 | 0.442 |
Rmeas | 0.132 | 0.468 |
Rpim | 0.041 | 0.154 |
Number of reflections | 10924 | 1077 |
<I/σ(I)> | 16.1 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.9 | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.8 M Ammonium citrate tribasic |