6J2P

Crystal structure of Saccharomyces cerevisiae Spp1 in complex with H3K4me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	81.810, 106.170, 139.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.279 - 2.850
R-factor	0.2372
Rwork	0.234
R-free	0.28780
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.574
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	69.915	46.610	3.000
High resolution limit [Å]	2.850	9.010	2.850
Rmerge		0.042	0.689
Rmeas	0.132	0.047	0.764
Rpim	0.056	0.020	0.324
Total number of observations	77725	2446	11497
Number of reflections	14530	494	2085
<I/σ(I)>	8.8	18.7	2.1
Completeness [%]	99.7	95	99.9
Redundancy	5.3	5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M ammonium sulfate, 20% PEG3350, 0.1 M HEPES ph 7.5