6J2P
Crystal structure of Saccharomyces cerevisiae Spp1 in complex with H3K4me3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.810, 106.170, 139.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.279 - 2.850 |
| R-factor | 0.2372 |
| Rwork | 0.234 |
| R-free | 0.28780 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.574 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 69.915 | 46.610 | 3.000 |
| High resolution limit [Å] | 2.850 | 9.010 | 2.850 |
| Rmerge | 0.042 | 0.689 | |
| Rmeas | 0.132 | 0.047 | 0.764 |
| Rpim | 0.056 | 0.020 | 0.324 |
| Total number of observations | 77725 | 2446 | 11497 |
| Number of reflections | 14530 | 494 | 2085 |
| <I/σ(I)> | 8.8 | 18.7 | 2.1 |
| Completeness [%] | 99.7 | 95 | 99.9 |
| Redundancy | 5.3 | 5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M ammonium sulfate, 20% PEG3350, 0.1 M HEPES ph 7.5 |
