6J1Y
Semi-open conformation E3 ligase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 60.019, 59.370, 85.075 |
Unit cell angles | 99.39, 92.29, 108.75 |
Refinement procedure
Resolution | 42.572 - 2.550 |
R-factor | 0.2104 |
Rwork | 0.208 |
R-free | 0.25430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xmc |
RMSD bond length | 0.008 |
RMSD bond angle | 0.935 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.108 | 0.800 |
Number of reflections | 33955 | 3493 |
<I/σ(I)> | 32.6 | 2.3 |
Completeness [%] | 95.2 | 92.3 |
Redundancy | 4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.1M Sodium malonate pH 5.0, 12% w/v Polyethyleneglycol 3350 |