6J1U
influenza virus nucleoprotein with a specific inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97930 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 119.592, 126.747, 194.425 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.530 - 2.800 |
R-factor | 0.19499 |
Rwork | 0.191 |
R-free | 0.25971 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2iqh |
RMSD bond length | 0.012 |
RMSD bond angle | 1.589 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.930 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.118 | 0.040 |
Number of reflections | 39939 | 3247 |
<I/σ(I)> | 13.9 | 2.6 |
Completeness [%] | 92.3 | 94.6 |
Redundancy | 6.5 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1 M sodium acetate, 0.05 M magnesium acetate, 0.1 M MES, pH 6.0, 7% PEG8000 and 5% glycerol |