6J1C

Photoswitchable fluorescent protein Gamillus, N150C/T204V double mutant, off-state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-12-17
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	I 21 3
Unit cell lengths	162.021, 162.021, 162.021
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.770 - 2.090
R-factor	0.162
Rwork	0.161
R-free	0.17690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y00
RMSD bond length	0.020
RMSD bond angle	2.253
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.772	46.772	2.220
High resolution limit [Å]	2.090	6.230	2.090
Rmerge	0.071	0.028	0.511
Rmeas	0.076	0.030	0.551
Total number of observations	305306
Number of reflections	41594	1660	6665
<I/σ(I)>	19.44	51.46	3.54
Completeness [%]	99.9	99	99.8
Redundancy	7.34	7.067	7.271
CC(1/2)	0.999	0.999	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	Ammonium phosphate, sodium citrate, sodium chloride, HEPES, pH5.0