6J1B

Photoswitchable fluorescent protein Gamillus, N150C/T204V double mutant, on-state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-12-11
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	I 21 3
Unit cell lengths	161.492, 161.492, 161.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.620 - 1.990
R-factor	0.1435
Rwork	0.143
R-free	0.16020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y00
RMSD bond length	0.031
RMSD bond angle	2.709
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.619	46.619	2.120
High resolution limit [Å]	1.990	5.940	1.990
Rmerge	0.071	0.032	0.254
Rmeas	0.073	0.034	0.264
Total number of observations	685896
Number of reflections	47626	1903	7653
<I/σ(I)>	27.64	57.62	8.75
Completeness [%]	100.0	99.7	100
Redundancy	14.402	14.107	13.78
CC(1/2)	0.999	1.000	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	mmonium phosphate, sodium citrate, sodium chloride, HEPES, pH5.0