6IZ4
Crystal Structure Analysis of TRIC counter-ion channels in calcium release
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-17 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.9778 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 290.246, 290.246, 195.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.932 - 3.098 |
R-factor | 0.2834 |
Rwork | 0.283 |
R-free | 0.30050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6iz5 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.2) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14rc2_3191) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.932 | 49.930 | 3.150 |
High resolution limit [Å] | 3.098 | 16.970 | 3.100 |
Rmerge | 0.389 | 0.040 | 4.774 |
Rmeas | 0.394 | 0.040 | 4.841 |
Rpim | 0.065 | 0.008 | 0.792 |
Number of reflections | 137600 | 1028 | 7391 |
<I/σ(I)> | 12 | ||
Completeness [%] | 91.0 | 95.8 | 99.5 |
Redundancy | 35.9 | 27.6 | 36.6 |
CC(1/2) | 0.999 | 0.999 | 0.463 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 278 | 22% PEG 400, 50mM MgCl2, 100mM Glycine pH 9.0 |