6IZ3

Structural basis for activity of TRIC counter-ion channels in calcium release

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-21
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.7712
Spacegroup name	P 3 2 1
Unit cell lengths	190.124, 190.124, 117.587
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.845 - 3.791
R-factor	0.3007
Rwork	0.299
R-free	0.34110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iz5
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	PHENIX
Refinement software	PHENIX (1.14rc2_3191)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.850	47.850	4.040
High resolution limit [Å]	3.780	10.700	3.780
Rmerge	0.237	0.079	7.723
Rmeas	0.241	0.080	7.834
Rpim	0.039	0.014	1.293
Number of reflections	24627	1165	4296
<I/σ(I)>	9.8
Completeness [%]	99.3	98.9	96.5
Redundancy	37.5	34.5	36.6
CC(1/2)	0.999	0.999	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	PEG 4000, KNO3 K-Citrate PH6.5