6IYY

Crystal structure of human WIPI3,loop deletion mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9785
Spacegroup name	C 2 2 21
Unit cell lengths	46.297, 104.931, 124.882
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.357 - 1.796
R-factor	0.1681
Rwork	0.165
R-free	0.20410
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.12_2829: ???)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.840
High resolution limit [Å]	1.796	1.800
Rmerge	0.065	0.230
Number of reflections	28765	1811
<I/σ(I)>	10.2	3
Completeness [%]	99.6	99.6
Redundancy	6.5	2.7
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	289	1.6M Ammonium Sulfate, 0.1M Tris-HCl