6IY7
E. coli peptide deformylase crystal structure fitted into the cryo-EM density map of E. coli 70S ribosome in complex with peptide deformylase
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | FEI EAGLE (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 10.5 |
Software | SPIDER |
CTF correction | PHASE FLIPPING ONLY |