6IV4
Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae with a mutation W252F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.009, 154.186, 161.736 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.930 - 3.140 |
| R-factor | 0.2432 |
| Rwork | 0.237 |
| R-free | 0.29460 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.217 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.930 | 3.250 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Number of reflections | 45665 | |
| <I/σ(I)> | 8.77 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05M zinc acetate, 6% v/v ethylene glycol, 0.1M sodium cacodylate, pH 6.0, 6.6% w/v PEG 8000 |
