6IUY

Structure of DsGPDH of Dunaliella salina

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-03
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.000021
Spacegroup name	P 41 2 2
Unit cell lengths	91.335, 91.335, 348.319
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.362 - 2.200
R-factor	0.2014
Rwork	0.199
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wpq
RMSD bond length	0.008
RMSD bond angle	1.183
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.362	47.362	2.330
High resolution limit [Å]	2.200	6.550	2.200
Rmerge	0.161	0.030	2.160
Rmeas	0.164	0.031	2.201
Total number of observations	3988632
Number of reflections	142895	5405	23022
<I/σ(I)>	21.82	111.36	1.69
Completeness [%]	99.9	99.8	99.6
Redundancy	27.913	28.476	27.453
CC(1/2)	0.999	1.000	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293.15	10 mM NAD+, 10 mM DHAP, 56% Tacsimate pH 7.5 (Hampton)