6IUL

Crystal structure of Lamprey immune protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-20
Detector	PSI PILATUS 6M
Wavelength(s)	0.9798
Spacegroup name	P 43 21 2
Unit cell lengths	103.257, 103.257, 166.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.250
R-factor	0.2
Rwork	0.198
R-free	0.23150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nzo
RMSD bond length	0.014
RMSD bond angle	1.496
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.000	45.000	2.290
High resolution limit [Å]	2.250	6.100	2.250
Rmerge	0.147	0.119	0.644
Rmeas	0.152	0.123	0.664
Rpim	0.038	0.033	0.162
Number of reflections	43291	2387	2116
<I/σ(I)>	6.7
Completeness [%]	99.9	99.6	100
Redundancy	16	13.8	16.3
CC(1/2)		0.993	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	289	2% 1,4-Dioxane, 10% w/v Polyethylene glycol 2000, 0.1M BICINE