6IUD
Structure of Helicobacter pylori Soj-ADP complex bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2017-05-01 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.773, 74.690, 80.749 |
| Unit cell angles | 71.54, 71.59, 67.73 |
Refinement procedure
| Resolution | 29.955 - 2.506 |
| R-factor | 0.2073 |
| Rwork | 0.205 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6iub |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.963 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.050 | |
| Number of reflections | 49136 | 5041 |
| <I/σ(I)> | 13.4 | 1.5 |
| Completeness [%] | 96.0 | 97.9 |
| Redundancy | 2 | 2.1 |
| CC(1/2) | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | MES (pH 5.6), KCl, MgCl2, PEG 8000 |
