6IUA
Crystal structure of importin-alpha1 bound to the 53BP1 nuclear localization signal (S1678D)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2018-11-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.260, 90.170, 97.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.324 - 1.700 |
R-factor | 0.1562 |
Rwork | 0.155 |
R-free | 0.17860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6iu7 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.7.3) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 24.530 | 24.530 | 1.730 |
High resolution limit [Å] | 1.700 | 9.000 | 1.700 |
Rmerge | 0.087 | 0.051 | 0.995 |
Rmeas | 0.094 | 0.057 | 1.080 |
Rpim | 0.037 | 0.024 | 0.416 |
Total number of observations | 493282 | ||
Number of reflections | 77094 | 502 | 4045 |
<I/σ(I)> | 11 | ||
Completeness [%] | 99.9 | 82.4 | 100 |
Redundancy | 6.4 | 5.1 | 6.6 |
CC(1/2) | 0.997 | 0.990 | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | sodium citrate, HEPES, DTT |