6IUA

Crystal structure of importin-alpha1 bound to the 53BP1 nuclear localization signal (S1678D)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2018-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	79.260, 90.170, 97.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.324 - 1.700
R-factor	0.1562
Rwork	0.155
R-free	0.17860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iu7
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.3)
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.530	24.530	1.730
High resolution limit [Å]	1.700	9.000	1.700
Rmerge	0.087	0.051	0.995
Rmeas	0.094	0.057	1.080
Rpim	0.037	0.024	0.416
Total number of observations	493282
Number of reflections	77094	502	4045
<I/σ(I)>	11
Completeness [%]	99.9	82.4	100
Redundancy	6.4	5.1	6.6
CC(1/2)	0.997	0.990	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	sodium citrate, HEPES, DTT