6IRY

Crystal structure of the zebrafish cap-specific adenosine methyltransferase bound to SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	71.379, 82.288, 92.811
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.623 - 1.800
R-factor	0.1802
Rwork	0.179
R-free	0.20340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6irx
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.623	46.620	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.040	0.046	0.587
Rmeas	0.044	0.050	0.635
Rpim	0.017	0.019	0.241
Number of reflections	51421	484	3019
<I/σ(I)>	21.2
Completeness [%]	100.0	99.5	100
Redundancy	6.7	6.1	6.8
CC(1/2)	0.999	0.997	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	13% PEG 3350, 0.1M potassium thiocyanate, 0.1M bis-Tris propane