6IPU

Human nucleosome core particle containing 145 bp of DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98.15
Detector technology	PIXEL
Collection date	2017-09-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	107.560, 109.710, 183.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.160 - 1.990
R-factor	0.237
Rwork	0.236
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nzd
RMSD bond length	0.008
RMSD bond angle	1.474
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.160	2.100
High resolution limit [Å]	1.990	1.990
Rpim	0.021	0.652
Number of reflections	148453	21030
<I/σ(I)>	17.4	1.3
Completeness [%]	99.6	97.9
Redundancy	12.2	11.6
CC(1/2)	0.997	0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291.15	manganese chloride, potassium chloride, potassium cacodylate