6IO4
Silver-bound Glyceraldehyde-3-phosphate dehydrogenase A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97914 |
Spacegroup name | P 1 |
Unit cell lengths | 87.991, 110.019, 139.712 |
Unit cell angles | 87.66, 86.94, 87.26 |
Refinement procedure
Resolution | 87.773 - 3.100 |
R-factor | 0.1779 |
Rwork | 0.175 |
R-free | 0.23970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gad |
Data reduction software | xia2 |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (2.7.2) |
Refinement software | PHENIX ((1.14_3260:001)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 139.410 | 139.410 | 3.180 |
High resolution limit [Å] | 3.100 | 13.860 | 3.100 |
Rmerge | 0.089 | 0.052 | 0.560 |
Rmeas | 0.105 | 0.061 | 0.655 |
Rpim | 0.055 | 0.032 | 0.338 |
Total number of observations | 306417 | 3252 | 23970 |
Number of reflections | 85684 | 967 | 6570 |
<I/σ(I)> | 8.5 | 18.2 | 2.1 |
Completeness [%] | 90.4 | 91.2 | 93.1 |
Redundancy | 3.6 | 3.4 | 3.6 |
CC(1/2) | 0.996 | 0.994 | 0.818 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M Malic acid, PEG3350 20% |