6IO4

Silver-bound Glyceraldehyde-3-phosphate dehydrogenase A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97914
Spacegroup name	P 1
Unit cell lengths	87.991, 110.019, 139.712
Unit cell angles	87.66, 86.94, 87.26

Refinement procedure

Resolution	87.773 - 3.100
R-factor	0.1779
Rwork	0.175
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gad
Data reduction software	xia2
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.2)
Refinement software	PHENIX ((1.14_3260:001))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	139.410	139.410	3.180
High resolution limit [Å]	3.100	13.860	3.100
Rmerge	0.089	0.052	0.560
Rmeas	0.105	0.061	0.655
Rpim	0.055	0.032	0.338
Total number of observations	306417	3252	23970
Number of reflections	85684	967	6570
<I/σ(I)>	8.5	18.2	2.1
Completeness [%]	90.4	91.2	93.1
Redundancy	3.6	3.4	3.6
CC(1/2)	0.996	0.994	0.818

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1M Malic acid, PEG3350 20%