6ILD
Crystal Structure of Human LysRS: P38/AIMP2 Complex II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 96.511, 100.074, 270.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.420 - 1.882 |
R-factor | 0.155 |
Rwork | 0.153 |
R-free | 0.18440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bju |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.950 |
High resolution limit [Å] | 1.880 | 4.050 | 1.880 |
Rmerge | 0.068 | 0.026 | 0.571 |
Number of reflections | 104488 | 10704 | 10197 |
<I/σ(I)> | 9.2 | ||
Completeness [%] | 98.5 | 97.2 | 97.6 |
Redundancy | 7.9 | 7.3 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 8 % PEG 20000, 0.1 M Tris pH 8.0, 0.1 M sodium chloride |