6IIK
USP14 catalytic domain with IU1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-09-01 |
Detector | OXFORD SAPPHIRE CCD |
Wavelength(s) | 0.984 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.869, 81.670, 107.371 |
Unit cell angles | 90.00, 93.68, 90.00 |
Refinement procedure
Resolution | 29.217 - 1.970 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.21810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ayn |
RMSD bond length | 0.008 |
RMSD bond angle | 1.208 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (phenix.refine: 1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 1.960 | 1.960 |
Number of reflections | 68318 | 6105 |
<I/σ(I)> | 28.25 | |
Completeness [%] | 96.7 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | NH4F. PEG 3350, glycine, Cscl |