6IGK
Crystal Structure of human ETB receptor in complex with Endothelin-3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-27 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 65.550, 172.280, 121.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.599 - 2.000 |
R-factor | 0.1853 |
Rwork | 0.183 |
R-free | 0.22710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5glh |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.599 | 49.599 | 2.120 |
High resolution limit [Å] | 2.000 | 5.990 | 2.000 |
Rmerge | 0.858 | 0.110 | 18.011 |
Rmeas | 0.860 | 0.110 | 18.057 |
Total number of observations | 9582405 | ||
Number of reflections | 46829 | 1879 | 7430 |
<I/σ(I)> | 20.7 | 96.91 | 0.95 |
Completeness [%] | 100.0 | 99.3 | 100 |
Redundancy | 204.625 | 207.283 | 199.228 |
CC(1/2) | 0.998 | 0.997 | 0.559 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6 | 293 | PEG 500DME, Ammonium citrate tribasic |