6IFJ
Structure of bispecific Fc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.660, 67.260, 78.730 |
| Unit cell angles | 90.00, 106.23, 90.00 |
Refinement procedure
| Resolution | 37.180 - 2.400 |
| R-factor | 0.174 |
| Rwork | 0.171 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4byh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.110 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.840 |
| Number of reflections | 23965 | 3485 |
| <I/σ(I)> | 5.6 | 0.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.5 | 3.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291.15 | 0.1M Hepes, pH7.5, 12%(w/v) polyethylene glycol 3350 |
