6IEU
The structure of TRIM66 PHD-Bromo domain with unmodified H3 N terminal peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.266, 63.465, 33.303 |
Unit cell angles | 90.00, 101.67, 90.00 |
Refinement procedure
Resolution | 32.614 - 1.787 |
R-factor | 0.1877 |
Rwork | 0.186 |
R-free | 0.21650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o33 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.886 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.614 | 1.851 |
High resolution limit [Å] | 1.787 | 1.787 |
Rmerge | 0.116 | 0.640 |
Number of reflections | 19103 | 1684 |
<I/σ(I)> | 22.409 | |
Completeness [%] | 98.3 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES sodium, pH 7.5,10% v/v 2-propanol, 20% PEG4000 |