6IET

The crystal structure of TRIM66 PHD-Bromo domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 61
Unit cell lengths	54.192, 54.192, 108.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.096 - 2.101
R-factor	0.2087
Rwork	0.206
R-free	0.25610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3o33
RMSD bond length	0.008
RMSD bond angle	0.839
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.096	2.176
High resolution limit [Å]	2.101	2.101
Rmerge	0.128	0.434
Number of reflections	10413	1026
<I/σ(I)>	34.33
Completeness [%]	99.0
Redundancy	20.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	30%(w/v)5000 MME, 100mM Tris base/ Hydrochloric acid pH 8.0, 200mM Lithium sulfate