6IEN
Substrate/product bound Argininosuccinate lyase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-01-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 114.180, 131.350, 145.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.860 - 2.700 |
| R-factor | 0.24887 |
| Rwork | 0.246 |
| R-free | 0.29295 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.338 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.860 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.257 | 0.812 |
| Number of reflections | 60527 | 8743 |
| <I/σ(I)> | 4.4 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 2.1M DL-malic acid pH 7.0, 10% v/v ethylene glycol |
