6IBD

The Phosphatase and C2 domains of Human SHIP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	62.889, 80.076, 90.273
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.900 - 1.480
R-factor	0.17385
Rwork	0.173
R-free	0.20054
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5okm
RMSD bond length	0.012
RMSD bond angle	1.791
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.080	1.510
High resolution limit [Å]	1.480	1.480
Rmerge	0.187	3.948
Rmeas	0.197	4.289
Rpim	0.062	1.639
Number of reflections	76674	3736
<I/σ(I)>	11.4	1.2
Completeness [%]	100.0	99.3
Redundancy	19.4	13.3
CC(1/2)	0.997	0.385

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M bis-tris, 14% (w/v) PEG 2000 MME, 12% (w/v) PEG 3350