6I9N

JmjC domain-containing protein 5 (JMJD5) in complex with Mn and L-2-hydroxyglutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91742
Spacegroup name	P 21 21 21
Unit cell lengths	49.642, 64.428, 77.543
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.808 - 1.361
R-factor	0.1501
Rwork	0.150
R-free	0.15890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gjz
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	HKL-2000 (706E)
Data scaling software	HKL-2000 (706E)
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.808	1.410
High resolution limit [Å]	1.340	1.340
Rmerge	0.081	0.529
Rmeas	0.086	0.561
Rpim	0.028	0.186
Number of reflections	53414	5302
<I/σ(I)>	24.125	4.2
Completeness [%]	98.8	99.4
Redundancy	9.6	8.9
CC(1/2)	0.997	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1 M Bis-Tris pH 6.5, 15.0 % PEG3350, 0.002 M MnCl2