6I9M

JmjC domain-containing protein 5 (JMJD5) in complex with Mn and R-2-hydroxyglutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.98000
Spacegroup name	P 21 21 21
Unit cell lengths	49.571, 64.674, 77.437
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.749 - 1.650
R-factor	0.1525
Rwork	0.151
R-free	0.18100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gjz
RMSD bond length	0.018
RMSD bond angle	1.400
Data reduction software	HKL-2000 (706E)
Data scaling software	HKL-2000 (706E)
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.749	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.108	0.788
Rmeas	0.118	0.861
Rpim	0.046	0.341
Number of reflections	30597	2984
<I/σ(I)>	18.17	3.18
Completeness [%]	99.8	99.2
Redundancy	6.7	6.2
CC(1/2)	0.995	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Bis-Tris pH 6.5, 15.0 % PEG3350, 0.002 M MnCl2