6I7R

Structure of pVHL-elongin B-elongin C (VCB) in complex with hydroxylated-HIF-2alpha (523-542) in the P43212 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 43 21 2
Unit cell lengths	61.460, 61.460, 261.815
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.454 - 1.949
R-factor	0.1745
Rwork	0.173
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lqb
RMSD bond length	0.007
RMSD bond angle	1.023
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	262.010	2.060
High resolution limit [Å]	1.949	1.949
Rmerge	0.175	1.945
Rmeas	0.178	1.985
Rpim	0.035	0.393
Number of reflections	38067	5457
<I/σ(I)>	14	2
Completeness [%]	100.0	100
Redundancy	24.9	25.2
CC(1/2)	0.999	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.8	293	0.1M Cacodyl-Na pH 5.8, 0.1M Mg Formate, 50mM Cystamine, 22% PEG3350