6I68
Co-crystal structure of human SPOP MATH domain (M117V) and human BRD3 fragment
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-23 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.775, 90.128, 88.563 |
| Unit cell angles | 90.00, 91.17, 90.00 |
Refinement procedure
| Resolution | 19.868 - 1.850 |
| R-factor | 0.1983 |
| Rwork | 0.196 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ivv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.823 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.130 | 4.750 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmeas | 0.054 | |
| Number of reflections | 70332 | 3410 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 200 mM Sodium formate, 100 mM Bis Tris propane pH 7.5, 20% (w/v) PEG 3350 |
