6I5U
Cryo-EM informed directed evolution of Nitrilase 4 leads to a change in quaternary structure.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 3.9 |
Number of Particle | 133106 |
Software | RELION,EPU,Gctf,PHENIX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |