6I5Q
Papaver somniferum O-methyltransferase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 112.004, 112.004, 304.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 97.000 - 3.050 |
| R-factor | 0.21247 |
| Rwork | 0.210 |
| R-free | 0.25569 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kyz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.597 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.000 | 3.170 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.151 | 1.607 |
| Rpim | 0.052 | 0.561 |
| Number of reflections | 43285 | 4464 |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.9 | 10 |
| CC(1/2) | 0.997 | 0.592 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | PEG 3350, sodium citrate, bis-tris propane |
