6I5E
X-ray structure of apo human soluble Epoxide Hydrolase C-terminal Domain (hsEH CTD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 88.224, 80.139, 104.696 |
| Unit cell angles | 90.00, 95.39, 90.00 |
Refinement procedure
| Resolution | 70.510 - 2.600 |
| R-factor | 0.24755 |
| Rwork | 0.246 |
| R-free | 0.27704 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ans |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.449 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.510 | 29.600 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.210 | 0.741 |
| Rmeas | 0.235 | 0.831 |
| Number of reflections | 22492 | |
| <I/σ(I)> | 5.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.077 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277.15 | 22.5% PEG 3350, 0.2 M malic acid |
