6I34
Crystal structure of Neanderthal glycine decarboxylase (P-protein)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.873, 124.381, 201.304 |
| Unit cell angles | 90.00, 98.57, 90.00 |
Refinement procedure
| Resolution | 47.900 - 2.100 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6i33 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.915 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.192 | |
| Number of reflections | 245944 | |
| <I/σ(I)> | 5.3 | 1.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.1 | 5.2 |
| CC(1/2) | 0.993 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M HEPES pH 7, 6 % PEG3350, 10 mM DTT |
