6I08
THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT E243C-I201W
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 191 |
Detector technology | PIXEL |
Collection date | 2016-11-25 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9677 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 180.800, 134.350, 159.350 |
Unit cell angles | 90.00, 101.83, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.21 |
Rwork | 0.209 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.120 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.900 | 3.060 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 73894 | |
<I/σ(I)> | 12.5 | 1.6 |
Completeness [%] | 99.0 | 99.8 |
Redundancy | 4.5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4 | 293 | PH 4, VAPOR DIFFUSION, TEMPERATURE 293K 12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4. |