6I04

Crystal structure of Sema domain of the Met receptor in complex with FAB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	73.932, 85.497, 101.300
Unit cell angles	77.49, 88.58, 68.76

Refinement procedure

Resolution	29.560 - 3.100
R-factor	0.20455
Rwork	0.202
R-free	0.26105
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4py7 4o3t
RMSD bond length	0.005
RMSD bond angle	1.368
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.570	3.230
High resolution limit [Å]	3.100	3.100
Rmerge	0.169	1.033
Number of reflections	39528	3994
<I/σ(I)>	5.2	1
Completeness [%]	96.9	86.1
Redundancy	3.6	3.1
CC(1/2)	0.987	0.446

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	18% w/v PEG 3350, 0.1 M ammonium citrate