6HZ1
THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT E243C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2016-05-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.984 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.900, 132.950, 160.060 |
| Unit cell angles | 90.00, 102.41, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.21600 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.070 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 122120 | |
| <I/σ(I)> | 8.2 | 1.5 |
| Completeness [%] | 95.6 | 95.7 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4 | 291 | 12-15% PEG4K, 0.1M NA ACETATE PH4, 0.4M NASCN, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K |
