6HYR
THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Q193C+MMTS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 193 |
Detector technology | PIXEL |
Collection date | 2015-11-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9660 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 178.984, 128.285, 160.429 |
Unit cell angles | 90.00, 100.96, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.500 |
R-factor | 0.13542 |
Rwork | 0.231 |
R-free | 0.24100 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.817 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.730 | 3.850 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 39511 | |
<I/σ(I)> | 6.4 | |
Completeness [%] | 99.3 | |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 291 | 12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4. |