6HYQ
Regulatory subunit of a cAMP-independent protein kinase A from Trypanosoma cruzi bound to guanosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.910, 80.819, 149.343 |
Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
Resolution | 59.839 - 2.080 |
R-factor | 0.262630893136 |
Rwork | 0.260 |
R-free | 0.31914 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.542 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.200 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmeas | 0.134 | |
Number of reflections | 166708 | |
<I/σ(I)> | 7.64 | |
Completeness [%] | 98.7 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | PEG 3350 (15 to 26%), Calcium acetate (0,05 to 0,4M), drop size: 500nL protein (10 mg/ml with 250 uM guanosine) +500nL buffer. Protein Purification Buffer (Size Exclusion Chromatography): 10mM Hepes pH 7.0, 50 mM NaCl, 1% DMSO, 1mM DTT, *The 6His tag was cleaved for crystallisation |