6HYG
Heteromeric tandem IgG4/IgG1 Fc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-08 |
Detector | MARRESEARCH |
Wavelength(s) | 0.972 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 136.119, 63.253, 77.551 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.280 - 2.310 |
R-factor | 0.25858 |
Rwork | 0.258 |
R-free | 0.27719 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c2s |
RMSD bond length | 0.019 |
RMSD bond angle | 2.398 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.280 | 2.390 |
High resolution limit [Å] | 2.310 | 2.310 |
Number of reflections | 27962 | |
<I/σ(I)> | 8.4 | |
Completeness [%] | 95.7 | |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 5 mM zinc acetate, 100 mM sodium cacodylate pH 6.5, 9 % (w/v) polyethylene glycol 8000 |