6HY3
Three-dimensional structure of AgaC from Zobellia galactanivorans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 60.604, 101.498, 46.693 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.420 - 1.300 |
R-factor | 0.1503 |
Rwork | 0.149 |
R-free | 0.16892 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.944 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MoRDa |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.420 | 1.346 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 70662 | |
<I/σ(I)> | 11.66 | |
Completeness [%] | 98.7 | |
Redundancy | 3.74 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.1 M sodium malonate and 1% glycerol |