6HXU

Crystal structure of Human RHOB Q63L in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-21
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.969
Spacegroup name	P 2 21 21
Unit cell lengths	38.890, 61.460, 76.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.690 - 1.190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fv8
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.960	1.230
High resolution limit [Å]	1.190	1.190
Number of reflections	58942	5349
<I/σ(I)>	20
Completeness [%]	99.0
Redundancy	2
CC(1/2)	0.999	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	100 mM MES pH6 30% v/w PEG 6000