6HX3
PDX1.2/PDX1.3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-21 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 177.760, 177.760, 115.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 92.330 - 2.000 |
| R-factor | 0.2245 |
| Rwork | 0.223 |
| R-free | 0.24950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5K3V CHAINSAW model |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.774 |
| Data reduction software | MOSFLM (7.2.0) |
| Data scaling software | Aimless (0.5.25) |
| Phasing software | PHASER (2.6.1) |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.330 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.209 | 3.698 |
| Rmeas | 0.235 | 4.173 |
| Rpim | 0.106 | 1.913 |
| Number of reflections | 118755 | 5897 |
| <I/σ(I)> | 7.7 | 0.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.6 | 9.5 |
| CC(1/2) | 0.997 | 0.129 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.8 M Ammonium sulphate, 0.05 M MES monophydrate pH 6.5, 5 % 1,4 Dioxane 10 mM Tris pH6.8, 100 mM KCl, 3 mM DTT |
