6HT9
Mouse fetuin-B in complex with crayfish astacin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979182 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.400, 85.800, 168.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.120 - 3.100 |
| R-factor | 0.217 |
| Rwork | 0.216 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ast |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.400 | 3.280 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.153 | 1.499 |
| Rmeas | 0.160 | 1.567 |
| Number of reflections | 22647 | |
| <I/σ(I)> | 14.1 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.7 | 11.6 |
| CC(1/2) | 0.998 | 0.771 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | ammonium sulfate, polyethylene glycol 2000, sodium acetate, pH 4.6. |
