6HRR

Structure of the TRPML2 ELD at pH 6.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9677
Spacegroup name	I 4 2 2
Unit cell lengths	109.471, 109.471, 149.744
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.188
Rwork	0.186
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tja
RMSD bond length	0.010
RMSD bond angle	1.020
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	Aimless (0.7.2)
Phasing software	PHASER (2.8.2)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.530	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.102	3.556
Rpim	0.002	0.673
Number of reflections	31030	2240
<I/σ(I)>	21.5	1.3
Completeness [%]	99.9	100
Redundancy	27.2	28.8
CC(1/2)	1.000	0.645

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	30% Jeffamine M-600 0.1 M MES pH 6.5 50 mM CsCl